Artificial cerebrospinal fluid, 20x concentrate (ACSF)

Artificial cerebrospinal fluid, 20x concentrate (ACSF)

Product no.: AN001396

Delivery period: 7 working days

42.00 / set(s)
Price plus VAT, plus delivery
Delivery weight: 1.7 kg

Artificial cerebrospinal fluid (aCSF) is a biological buffer that enables a vital environment for neuronal tissue by maintaining the homeostasis, osmolarity, and pH at physiological levels. ACSF is commonly used for many lab experiments including in vitro and in vivo applications.

20x concentrate, autoclaved

2 Components, Set of 2x 500ml

Instructions for use:

For 1000 ml ACSF Solution:

1. Pour 50 ml Base A (20x) into a flask and add 900 ml deionized water

2. Add slowly 50 ml Base B (20x)

3. Oxygenate ACSF Solution during use


pH 7.4 when oxygenated with 95% O2, 5% CO2 (Carbogene)

Use immediately once opened. Store at 4 - 8 °C


Ional composition of ACSF Solution (Base A + Base B)

NaCl = 125 mM

KCl = 3 mM

CaCl2 = 2.5 mM

MgSO4 = 1.3 mM

NaH2PO4 = 1.25 mM

NaHCO3 = 26 mM

Glucose = 13 mM

Additional product information

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Browse this category: Physiological Media