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Delivery period: 7 working days
Calcium-free Modified Barth's Solution (CMBS) is used for defolliculation of Xenopus laevis oocytes by collagenase treatment.
10x concentrate, autoclaved
Instructions for use:
For 1000 ml CMBS Solution:
1. Pour 100 ml CMBS (10x) into a flask
2. Add deionized water to a final volume of 1000 ml
Adjust to pH 7.4
Use immediately once opened. Store at 4 - 8 °C
Ional composition of CMBS Solution
NaCl = 88 mM
KCl = 1 mM
MgSO4 = 0,8 mM
TRIS-HCl = 5 mM
NaHCO3 = 2,4 mM