Calcium-free Barth's Solution,10x concentrate (CMBS)

Calcium-free Modified Barth's Solution (CMBS) is used for defolliculation of Xenopus laevis oocytes by collagenase treatment.

10x concentrate, autoclaved

Instructions for use:

For 1000 ml CMBS Solution:

1. Pour 100 ml CMBS (10x) into a flask

2. Add deionized water to a final volume of 1000 ml

Adjust to pH 7.4

Use immediately once opened. Store at 4 - 8 °C

Ional composition of CMBS Solution

NaCl = 88 mM

KCl = 1 mM

MgSO4 = 0,8 mM

TRIS-HCl = 5 mM

NaHCO3 = 2,4 mM